

Tinshemet cave during the excavations. (Credit- Yossi Zaidner)
In a nutshell
- Different human species shared identical cultural practices 100,000 years ago, suggesting social interaction and cultural exchange were common despite physical differences.
- Archaeologists found consistent burial positions, toolmaking techniques, and ochre use across sites in the Levant region, representing the world’s earliest evidence of intentional human burial.
- These findings challenge traditional views of human evolution, suggesting what makes us human may be our capacity for cultural exchange rather than our biological differences.
JERUSALEM — In a limestone cave in Israel, archaeologists have uncovered evidence of what might be the oldest case of cultural sharing between different human species. The discovery reveals that around 100,000 years ago, early Homo sapiens and their Neanderthal-like neighbors weren’t just occasionally bumping into each other—they were participating in a shared cultural world, complete with identical toolmaking traditions, hunting practices, and even burial rituals. This finding turns the traditional story of human evolution on its head, suggesting that cultural exchange between different human species was the rule, not the exception, in our ancient past.
The findings at Tinshemet Cave, published in Nature Human Behaviour, provide a rare glimpse into a pivotal period when multiple human species coexisted in the Middle East. The site has yielded fully articulated human skeletons carefully positioned in burial positions, thousands of ochre fragments transported from distant sources, stone tools made with consistent manufacturing techniques, and animal bones that reveal specific hunting preferences—all dating to what scientists call the mid-Middle Paleolithic period (130,000-80,000 years ago).
“Our data show that human connections and population interactions have been fundamental in driving cultural and technological innovations throughout history,” says lead researcher Prof. Yossi Zaidner of the Hebrew University in Jerusalem, in a statement.
The discovery is especially significant because the Levant region (modern-day Israel, Lebanon, Syria, and Jordan) served as a crossroads where different human populations met. Previous discoveries in the region had uncovered fossils with mixed physical characteristics, suggesting that interbreeding occurred between Homo sapiens migrating out of Africa and local Neanderthal-like populations.


What makes the Tinshemet Cave findings transformative is that they demonstrate these different-looking humans weren’t just meeting and mating—they were sharing their unique cultural behaviors and traditions across population boundaries.
Located just 10 kilometers from another significant archaeological site called Nesher Ramla (where Neanderthal-like fossils were previously discovered), Tinshemet Cave preserves evidence of sustained human occupation over thousands of years. The research team excavated multiple layers of sediments inside the cave and on its terrace, uncovering a wealth of artifacts that tell a cohesive story of sophisticated human activity.
Among the most striking discoveries are the human burials. The excavations revealed at least five individuals, including two complete articulated skeletons—one adult and one child. The bodies were deliberately placed in a fetal position on their sides with bent limbs, a burial position remarkably similar to contemporaneous burials found at other Middle Paleolithic sites in the region, including the famous Qafzeh and Skhul caves.
These burials represent the earliest known examples of intentional human burial anywhere in the world, predating similar practices in Europe and Africa by tens of thousands of years. More importantly, they show that diverse human populations were treating their dead with similar ceremonial care, suggesting shared symbolic behaviors and possibly shared beliefs.


Another fascinating discovery was the abundant presence of ochre—a naturally occurring mineral pigment that produces red, yellow, and purple hues. The research team recovered more than 7,500 ochre fragments throughout the site, with the highest concentrations found in layers containing human burials. Chemical analysis revealed that these ochre materials came from at least four different sources, some located as far as 60-80 kilometers away in Galilee, and others possibly from the central Negev, more than 100 kilometers to the south.
The significant effort invested in obtaining these pigments from distant sources suggests their importance in the lives of these ancient people. The presence of large chunks of ochre near human remains—including a 4-5 cm piece found between the legs of one buried individual—hints at their ritual significance. Evidence of heat treatment to enhance the red color of some ochre pieces further reveals sophisticated knowledge and intentional manipulation of these materials.
Stone tool production at Tinshemet Cave demonstrates another dimension of cultural uniformity. The researchers analyzed nearly 2,800 stone artifacts and found that a specific flint-knapping technique known as the centripetal Levallois method dominated tool production. This method, which involves careful preparation of a stone core to produce standardized flakes, appears consistently across mid-Middle Paleolithic sites in the region.
This technological consistency is particularly remarkable because it differs significantly from both earlier and later stone tool traditions in the Levant. Earlier Middle Paleolithic populations (around 250,000-140,000 years ago) primarily used methods to produce blade-like tools, while later populations (after 80,000 years ago) employed a more diverse set of techniques. The dominance of the centripetal Levallois method during this middle period represents a distinct technological tradition shared across populations.


Analysis of animal bones from the site reveals a third element of behavioral uniformity: a focus on hunting large game animals. Unlike earlier and later periods, when smaller prey like gazelles dominated the diet, the mid-Middle Paleolithic hunters at Tinshemet and similar sites showed a clear preference for larger ungulates, particularly aurochs (wild cattle) and equids (horse-like animals). This pattern suggests either a shift in hunting strategies or different approaches to transporting animal resources, possibly connected to changes in settlement patterns.
To establish the age of the findings, the research team employed multiple dating techniques, including thermoluminescence dating of burnt flint, optically stimulated luminescence dating of quartz grains in the sediments, and uranium-series dating of snail shells and flowstones. These methods consistently dated the main human occupation layers to approximately 97,000-106,000 years ago, placing them firmly within the mid-Middle Paleolithic period.
The timing corresponds to a warm interglacial period known as Marine Isotope Stage 5, when climatic conditions in the Levant were relatively favorable. Pollen analysis from the lowest layers of the cave indicates a Mediterranean open forest environment with wide-spaced trees, small shrubs, and herbs dominated by evergreen oak.


Perhaps most intriguing about the Tinshemet Cave discovery is what it suggests about interactions between different human populations. “These findings paint a picture of dynamic interactions shaped by both cooperation and competition,” says co-lead author Dr. Marion Prévost.
Scientists have long associated specific behaviors or technologies exclusively with particular human species. Now we have strong evidence that points to a landscape of interaction, where cultural innovations spread across population boundaries through social learning and exchange.
As excavations at Tinshemet Cave continue, researchers hope to uncover additional evidence about the lives and interactions of these ancient people. The site has already yielded remarkable insights into a crucial chapter of human prehistory—a time when different human populations met, exchanged ideas, and created shared traditions despite their physical differences. What began as a simple archaeological survey has evolved into a profound reconsideration of what it means to be human, showing that cultural connections can transcend biological boundaries.


Paper Summary
Methodology
The research at Tinshemet Cave involved excavation of multiple areas, including the cave’s terrace and its first chamber. Researchers removed sediments in 5-centimeter-thick layers within half-meter square units, recording the exact location of every significant artifact using a total station—a surveying instrument that captures precise spatial coordinates. Due to the hardened, cemented nature of many sediments, the team used specialized tools including pneumatic devices, grinders, electric hammers, and hand chisels, often softening the deposits with water first. This careful approach allowed them to extract complete artifacts without damage and maintain precise spatial control. For dating the site, researchers employed multiple independent techniques. They used thermoluminescence (TL) dating on burnt flint, which measures the time since the stone was last heated; optically stimulated luminescence (OSL) on quartz grains from the sediments, which determines when minerals were last exposed to sunlight; and uranium-series dating on snail shells and flowstones found in the deposits. The team also analyzed volcanic ash particles to correlate different layers across the site. Other specialized analyses included mineral identification of sediments, microscopic examination of thin sections, analysis of small mammal remains, identification of pollen for understanding ancient environments, and detailed studies of stone tools, ochre composition, and animal bones.
Results
The dating methods consistently placed the main human occupation layers of Tinshemet Cave between 97,000 and 106,000 years ago, with TL dating of burnt flints yielding a mean age of 96.3 ± 7.2 ka (thousand years ago) for Layer III in the First Chamber and 98.3 ± 8.0 ka for the Terrace. OSL dating produced similar results, with a mean age of 106.5 ± 16 ka for the Terrace Unit A layers and 114 ± 8 ka for Layer III in the First Chamber. Uranium-series dating of snail shells provided complementary ages of 113.3 ± 3.3 ka, 105.5 ± 4.1 ka, and 138.9 ± 6.4 ka. Analysis of the stone tools revealed a strong emphasis on the centripetal Levallois method, with nearly identical patterns in both the Terrace and First Chamber areas. Of the 2,780 stone artifacts analyzed, only a small percentage (1.3% on the Terrace and 3.1% in the First Chamber) were retouched tools, suggesting that the inhabitants primarily used unmodified flakes. The more than 7,500 ochre fragments discovered were classified into six different types based on grain size, morphology, and composition, with red to orange hues dominating (75.7%). Analysis of 191 identified animal bone specimens showed that ungulates comprised 88% of the assemblage, with aurochs, equids, fallow deer, and gazelle nearly equally represented. The human remains included two fully articulated skeletons and three isolated skulls, with all articulated individuals buried in the same fetal or sleeping position—lying on their sides with flexed legs and arms bent toward the chest and face.
Limitations of the Study
The researchers acknowledge several limitations to their study. The cemented nature of many sediments made excavation challenging and might have affected the recovery of smaller artifacts. While the site yielded rich archaeological materials, disturbance by insects and plant roots was observed in some areas, potentially causing minor movement of smaller items within the deposits. The faunal sample size (191 specimens) was relatively small, limiting quantitative analysis of hunting patterns. Additionally, analysis of how animal remains became fossilized has yet to be fully completed, making it difficult to determine with certainty whether humans were the primary accumulation agent for all faunal remains. The human fossils discovered at Tinshemet are still under study and are only preliminarily characterized as Homo spp., without definitive species assignment. Finally, while the research team proposes that behavioral uniformity resulted from intensified interactions between different human populations, direct genetic evidence from the Tinshemet human remains is not yet available to confirm admixture between populations at this specific site.
Discussion and Takeaways
The Tinshemet Cave findings transform our understanding of human evolution during the mid-Middle Paleolithic period. Rather than viewing different human groups as isolated populations with distinct behaviors, the evidence suggests a complex landscape of interaction where cultural innovations spread across population boundaries through social learning and exchange. The researchers emphasize that this period of behavioral uniformity contrasts sharply with both earlier and later periods, which show greater technological diversity. The consistent burial practices discovered at Tinshemet, Qafzeh, and Skhul represent the earliest known examples of intentional human burial worldwide, predating similar practices in Europe and Africa by tens of thousands of years. This suggests that complex symbolic behaviors emerged earlier than previously thought and were shared across morphologically diverse populations. The researchers propose that this cultural uniformity may have facilitated successful human adaptations during early waves of migration out of Africa, with the Levant serving as a crucial stepping stone for wider human dispersal into Asia. Overall, the study challenges traditional narratives about human evolution that associate specific behaviors exclusively with particular human species, instead suggesting that what defines humanity may emerge through social interactions and cultural exchanges that transcend biological boundaries.
Funding and Disclosures
The research was supported by multiple organizations, including the Israel Science Foundation (grants 2229/23, 1936/18, 1458/19, and 1084/23), the Dan David Foundation, the Research Authority at the Hebrew University of Jerusalem, the Leakey Foundation, the National Geographic Society (grants NGS-72344R-20 and NGS-51135R-18), the Gerda Henkel Foundation, the Wenner-Gren Foundation, and the Irene Levi Sala Care Archaeological Foundation. The Israel Nature and Parks Authority provided support during fieldwork. The researchers declared no competing interests that might have influenced the study’s findings or interpretations.
Publication Information
The study, titled “Evidence from Tinshemet Cave in Israel suggests behavioural uniformity across Homo groups in the Levantine mid-Middle Palaeolithic circa 130,000–80,000 years ago,” was published in the journal Nature Human Behaviour on March 11, 2025. The research was led by Yossi Zaidner of the Institute of Archaeology at the Hebrew University of Jerusalem, with contributions from 34 co-authors representing institutions in Israel, France, the United Kingdom, Switzerland, and Italy. The paper can be accessed online at https://doi.org/10.1038/s41562-025-02110-y.